How to Analyze Real time PCR Data? | Real Time PCR Gene Expression Fold Change Calculation
Data File used for demonstration: [Data File Link](https://docs.google.com/spreadsheets/...)
Welcome to my channel, "Learn Innovative with Shashi Bhushan Chauhan". In today's video, we delve into the nitty-gritty of Real-Time PCR Data Analysis and Fold Change Calculation, simplifying it for researchers and students alike.
In the realm of molecular biology, real-time PCR has become a cornerstone for quantifying gene expression. However, interpreting these results can often be challenging, particularly when calculating the fold-change gene expression data. This tutorial is designed to dispel those difficulties.
I will guide you through the process of calculating the fold change gene expression from real-time PCR data, using both Syber Green and Taqman Assays. This video addresses common obstacles faced by many research students, particularly when handling more than one control sample in their studies.
In this step-by-step tutorial, I'll show you how to calculate mean CT value, ΔCT value, ΔΔCT value, and finally, the fold change. I will demonstrate this process using Excel, making it a breeze to follow along.
In addition to this, I'll showcase how to represent your data visually, using graphs on Excel and the GraphPad Prism software, including creating an informative heat map.
Data File used for demonstration: [Data File Link](https://docs.google.com/spreadsheets/...)
This video is part of a series designed to make complex scientific processes accessible and understandable. So whether you're a seasoned researcher or a student starting in the field, this tutorial has something for you. If you find this video helpful, please like, share, and subscribe to support the channel.
Thank you for watching!
Key word for search:
#How to Calculate Fold change for Real-time PCR #Calculate 2^-delta delta Ct using Excel #How To Perform The Delta-Delta Ct Method #How to calculate delta delta Ct in Excel #Analysis of Real Time PCR (qRT-PCR) data: a ∆∆ct Method #Real Time QPCR Data Analysis Tutorial
#Real Time QPCR Data Analysis Tutorial #How to Analyze Real-time PCR Data #How to Analyze Real-time PCR Data #How to Analyze Real-time PCR Data #Real Time PCR Analysis qPCR Terms
Time Code:
00:00 - Introduction
00:48 - Summary of all steps
01:03 - Calculation of Mean Ct value of each sample
02:20 - Calculation of delta Ct value
03:06 - Calculation of delta delta Ct value
04:20 - Fold Change Gene expression calculation
06:16 - Fold Change gene expression Graph in Excel
06:36 - Fold Change gene expression graph in Graph Pad Prism Software & Export
Welcome to my channel, "Learn Innovative with Shashi Bhushan Chauhan". In today's video, we delve into the nitty-gritty of Real-Time PCR Data Analysis and Fold Change Calculation, simplifying it for researchers and students alike.
In the realm of molecular biology, real-time PCR has become a cornerstone for quantifying gene expression. However, interpreting these results can often be challenging, particularly when calculating the fold-change gene expression data. This tutorial is designed to dispel those difficulties.
I will guide you through the process of calculating the fold change gene expression from real-time PCR data, using both Syber Green and Taqman Assays. This video addresses common obstacles faced by many research students, particularly when handling more than one control sample in their studies.
In this step-by-step tutorial, I'll show you how to calculate mean CT value, ΔCT value, ΔΔCT value, and finally, the fold change. I will demonstrate this process using Excel, making it a breeze to follow along.
In addition to this, I'll showcase how to represent your data visually, using graphs on Excel and the GraphPad Prism software, including creating an informative heat map.
Data File used for demonstration: [Data File Link](https://docs.google.com/spreadsheets/...)
This video is part of a series designed to make complex scientific processes accessible and understandable. So whether you're a seasoned researcher or a student starting in the field, this tutorial has something for you. If you find this video helpful, please like, share, and subscribe to support the channel.
Thank you for watching!
Key word for search:
#How to Calculate Fold change for Real-time PCR #Calculate 2^-delta delta Ct using Excel #How To Perform The Delta-Delta Ct Method #How to calculate delta delta Ct in Excel #Analysis of Real Time PCR (qRT-PCR) data: a ∆∆ct Method #Real Time QPCR Data Analysis Tutorial
#Real Time QPCR Data Analysis Tutorial #How to Analyze Real-time PCR Data #How to Analyze Real-time PCR Data #How to Analyze Real-time PCR Data #Real Time PCR Analysis qPCR Terms
Time Code:
00:00 - Introduction
00:48 - Summary of all steps
01:03 - Calculation of Mean Ct value of each sample
02:20 - Calculation of delta Ct value
03:06 - Calculation of delta delta Ct value
04:20 - Fold Change Gene expression calculation
06:16 - Fold Change gene expression Graph in Excel
06:36 - Fold Change gene expression graph in Graph Pad Prism Software & Export
همه توضیحات ...